Correlation analysis and benchmark dose study on bone metabolic biochemical index of low doses of exposed hydrogen fluoride workers / 中华劳动卫生职业病杂志

Objective: To analyze correlation of occupational hydrogen fluoride exposure to low doses of bone metabolism index through occupational epidemiological investigation and benchmark dose calculation. Methods: In May 2021, using cluster sampling method, 237 workers exposed to hydrogen fluoride in a company were selected as the contact group, and 83 workers not exposed to hydrogen fluoride in an electronics production company were selected as the control group. The external exposure dose and urinary fluoride concentration, blood and urine biochemical indicators of the workers was measured.The relationship between external dose and internal dose of hydrogen fluoride was analyzed. The external dose, urinary fluoride was used as exposure biomarkers, while serum osteocalcin (BGP), serum alkaline phosphatase (AKP) and urinary hydroxyproline (HYP) were used as effect biomarkers for bone metabolism of hydrogen fluoride exposure. The benchmark dose calculation software (BMDS1.3.2) was used to calculate benchmark dose (BMD) . Results: Urine fluoride concentration in the contact group was correlated with creatinine-adjusted urine fluoride concentration (r=0.69, P=0.001). There was no significant correlation between the external dose of hydrogen fluoride and urine fluoride in the contact group (r=0.03, P=0.132). The concentrations of urine fluoride in the contact group and the control group were (0.81±0.61) and (0.45±0.14) mg/L, respectively, and the difference between the two groups was statistically significant (t=5.01, P=0.025). Using BGP, AKP and HYP as effect indexes, the urinary BMDL-05 values were 1.28, 1.47 and 1.08 mg/L, respectively. Conclusion: Urinary fluoride can sensitively reflect the changes in the effect indexes of biochemical indexes of bone metabolism. BGP and HYP can be used as early sensitive effect indexes of occupational hydrogen fluoride exposure.

Animal experimental study on the effects of different levels of amputation on cardiovascular system / 生物医学工程学杂志

Vascular injury resulting from lower limb amputation leads to the redistribution of blood flow and changes in vascular terminal resistance, which can affect the cardiovascular system. However, there was no clear understanding of how different amputation levels affect the cardiovascular system in animal experiments. Therefore, this study established two animal models of above-knee amputation (AKA) and below-knee amputation (BKA) to explore the effects of different amputation levels on the cardiovascular system through blood and histopathological examinations. The results showed that amputation caused pathological changes in the cardiovascular system of animals, including endothelial injury, inflammation, and angiosclerosis. The degree of cardiovascular injury was higher in the AKA group than in the BKA group. This study sheds light on the internal mechanisms of amputation's impact on the cardiovascular system. Based on the amputation level of patients, the findings recommend more comprehensive and targeted monitoring after surgery and necessary interventions to prevent cardiovascular diseases.

Analysis of Animal Models of Constipation Based on Characteristics of Clinical Symptoms of Traditional Chinese and Western Medicine / 中国实验方剂学杂志

Constipation is a common functional gastrointestinal disease with obstructive constipation， prolonged defecation time， or difficulty in defecation as the main symptoms， its prevalence is increasing year by year. Copying the animal model that is highly consistent with the clinical characteristics of traditional Chinese medicine （TCM） and western medicine is the basis for the related research on constipation. Based on the clinical characteristics， etiology and pathogenesis， clinical diagnostic criteria and TCM syndrome differentiation of constipation， a comparative analysis of the anastomosis between common animal models and clinical symptoms was carried out. Existing animal models of constipation were mostly drug induction and non-pharmaceutical intervention methods， and the modeling method was relatively simple， which was different from the multi-factors of constipation in clinical practice， and the evaluation indicators were mainly charcoal-powder propelling rate， defecation function， colon tissue and serum biochemical indicators. TCM syndrome differentiation lacked perfect evaluation system， such as laborious defecation， palpitation， shortness of breath， forgetfulness， cold pain in the abdomen， and other indicators that had not been embodied in the animal models， and the observation indicators were inconsistent with the diagnostic criteria of TCM. The same pathogenic factor could cause different syndromes， such as shortness of breath and fatigue， hot flashes and night sweats in the diagnostic criteria of TCM. Therefore， it is necessary to further explore the animal model of constipation that is highly consistent with the clinical characteristics of TCM and western medicine， in order to promote clinical treatment of constipation.

Study on the laboratory historical control values of biological indicators in SD rats with 28-day repeated dose oral toxicity tests / 中国职业医学

OBJECTIVE: To establish the laboratory historical control values for biological indicators in SD rats with 28-day repeated dose oral toxicity tests. METHODS: The body mass, blood routine indexes, serum biochemical indexes, organ mass and organ coefficient of 10 batches of specific pathogen free SD rats in the control group and the control additional group were collected for 28-day repeated dose oral toxicity tests, and the historical control values was established. RESULTS: The body mass of both male and female SD rats increased with the increasing age(all P<0.01). The body mass of male rats was higher than that of female rats each week(all P<0.01). The body mass, blood routine and serum biochemical indexes, organ mass and organ coefficient of SD rats were affected by the age and gender of rats to varying degrees. The effects of age and gender on organ mass and organ coefficient were not consistent. The laboratory historical control values of body mass, blood routine indexes, serum biochemical indexes, organ mass and organ coefficient of SD rats were established according to the age measured in weeks and the gender of rats. CONCLUSION: The laboratory control values of biological indicators of SD rats should be established according to different weekly age and the gender of rats. Organ coefficient is more suitable as an observation index for toxicological safety evaluation compared with organ mass.

Efficacy-toxicity Evaluation of Mahuang Xixin Fuzi Decoction on Mice with Kidney-yang Deficiency / 中国药学杂志

OBJECTIVE: To study the pharmacodynamic effects of Mahuang Xixin Fuzi Decoction (MXF) on mice with kidney-yang deficiency, its toxic effects on normal mice and effect-toxic mechanism. METHODS: BABL/C male mice were randomly divided into normal groups; normal groups with low, medium and high toxicity; kidney Yang deficiency model group. The low, medium and high dose groups were treated with infected Influenza virus of kidney-yang deficiency. After continuous gavage for 6 days, the body weight anal temperature and other pharmacological indicators were recorded, and the general signs, biochemical indicators and inflammatory factors of the mice were detected. RESULTS: Compared with the normal group, the biochemical parameters of the kidney yang deficiency model group, which means the creatine kinase (CK), creatine kinase-isoenzyme (CK-MB), alpha-hydroxybutyrate dehydrogenase (α-HBDH), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP) and urea (urea) were significantly increased (P<0.05), inflammatory factors interleukin-6 (IL-6), monocyte deproteinization protein (MCP-1), tumor necrosis factor-α (TNF-α), interleukin-10 (IL- 10), interferon-γ (INF-γ) and interleukin 12 (IL-12p70) increased significantly (P<0.05). After administration, the levels of biochemical markers such as CK, ALT and urea in each administration groups were slightly reduced compared with the model group. The levels of inflammatory factors such as IL-6, IL-10 and IFN-γ in each administration groups showed different degrees of callback compared with the model group. In the toxicity study, the biochemical indicators of CK, urea, and cysteine (CYS-C) in each drug-administered group were significantly higher than those in the normal group (P<0.05), and the inflammatory factor interleukin-10 (IL-10) increased significantly, and the content of interleukin-12 (IL-12p70) decreased significantly (P<0.05). CONCLUSION: After treatment with MXF, the above biochemical indicators of mice with kidney yang deficiency and external sensation have different degrees of callback. MXF can achieve therapeutic effects by regulating IL-6, IL-10, IFN-γ, MCP-1, TNF-α and other factors on the body′s inflammatory response. While normal mice give different doses of MXF, the heart, liver and kidney function are dysfunctional, the balance of immune response of the body are broken through cell transfer, thus making normal mouse developed an inflammatory response.

Studies on characteristics of liver injury model induced by rifampicin and isoniazid in mice / 中国药理学通报

Aim: To establish an ideal anti-tuberculosis drug induced liver injury model and provide a suitable animal model for its pharmacodynamic evaluation. Methods To explore the contribution rate and interaction of isoniazid (INH) and rifampicin (RIF) on liver injury, mice received intragastric administration of RIF 100 nig · kg-1, 300 mg · kg-1 once, or RIF 300 mg · kg-1, INH 150 mg · kg-1, and RIF 300 mg · kg-1 + INH 150 mg · kg-1 for 1 -3 weeks. Then the biochemical and pathological indexes were determined and analyzed by factorial design ANOVA. Results After single intragastric administration of rifampicin, the serum bilirubin levels gradually increased, but no other indicators were affected in mice. Continuous intragastric administration of RIF 300 mg · kg-1, INH 150 mg · kg-1 or RIF 300 mg · kg-1 +INH 150 mg · kg-1 for 1 ∼3 weeks could significantly increase the liver index of mice. RIF alone or combined with isoniazid could significantly increase the level of ALT, AST and TBIL, resulting in vacuolar lesions in liver tissues in mice. The analysis of variance demonstrated that the combined use of RIF and INH for two weeks or three weeks showed significant antagonism in liver index and the level of ALT, and marked antagonism in TBIL at three weeks. The pathological results were basically consistent with the biochemical indicators. Conclusions RIF is the leading cause of liver injury in mice, and its hepatotoxicity is related to cholestasis. INH has a significant antagonistic effect on liver toxicity of RIF when they are combined, and the deep action mechanisms remains to be further explored.

Study on the toxicology of traditional medicine Nao-de-kang / 中国基层医药

Objective@#To study the toxicological effects of Nao-de-kang on rats.@*Methods@#According to the digital table, SD rats were randomly divided into blank group and Nao-de-kang small dose group(3.85g/kg), high dose group(7.71g/kg) and maximum tolerated dose group(77.14g/kg), 20 rats in each group, half male and half female.All groups were treated for 12 weeks, and the maximum tolerated dose group was treated for 1 week.The animal activity during treatment, the blood routine indicators, blood biochemical index and the organs for pathological examination were recorded and compared.@*Results@#There was no mortality in the dose group of rats in the experimental period.The weights of 4 groups had no statistically significant differences during treatment(F=0.688, 0.540, 0.121, 0.065, 0.128, 0.239, 0.199, 0.378, 0.127, 0.446, 0.906 and 0.665, P=0.562, 0.585, 0.886, 0.937, 0.880, 0.788, 0.820, 0.687, 0.881, 0.642, 0.410 and 0.518), male and femal′s red blood cell(RBC)(♀: F=0.178, P=0.910, ♂: F=0.119, P=0.948), hemoglobin(HGB)(♀: F=0.046, P=0.987, ♂: F=0.072, P=0.975), hematokrit(HCT)(♀: F=0.126, P=0.944, ♂: F=0.054, P=0.983), platelet(PLT)(♀: F=0.515, P=0.675, ♂: F=0.500, P=0.685), white blood cell(WBC)(♀: F=0.078, P=0.972, ♂: F=0.057, P=0.982), eosnophils(Eos)(♀: F=0.078, P=0.972, ♂: F=0.057, P=0.982), lymphocyte(Lym)(♀: F=0.078, P=0.972, ♂: F=0.057, P=0.982), neutrophilic granulocyte(Neu)(♀: F=0.134, P=0.939, ♂: F=0.090, P=0.999), alanine aminotransfease (ALT)(♀: F=0.572, P=0.637, ♂: F=0.200, P=0.896), aspartate aminotransferase(AST)(♀: F=0.572, P=0.637, ♂: F=0.200, P=0.896), total protein(TP)(♀: F=0.665, P=0.579, ♂: F=0.343, P=0.795), albumin(ALB)(♀: F=0.533, P=0.663, ♂: F=0.668, P=0.577), triglyceride(TG)(♀: F=0.843, P=0.480, ♂: F=0.561, P=0.644), cholesterol(CHOL)(♀: F=0.245, P=0.864, ♂: F=0.046, P=0.987), glucose(GLU)(♀: F=0.216, P=0.884, ♂: F=0.095, P=0.963), urea nitrogen(BUN)(♀: F=0.172, P=0.914, ♂: F=0.203, P=0.894)and creatinine(Cr)(♀: F=0.172, P=0.914, ♂: F=0.203, P=0.894) had no statistically significant differences.@*Conclusion@#Nao-de-kang has no obvious side effect.

Effects of continuous renal replacement therapy on biochemical and physiological parameters of severe acute pancreatitis / 国际检验医学杂志

Objective To investigate the effects of continuous renal replacement therapy (CRRT) on biochemical and physiological indexes of severe acute pancreatitis (SAP) and its clinical efficacy.Methods Totally 98 patients with severe acute pancreatitis admitted in our hospital were divided into experimental group(n=49) and control group(n=49).The experimental group received CRRT and the control group did not receive.Heart rate,body temperature,blood routine,urine output,mean arterial pressure,liver function,blood biochemistry and arterial blood gas analysis before and after treatment in the two groups were measured and compared before and after treatment.Results In the experimental group,respiration,heart rate,oxygen partial pressure (PO2),carbon dioxide partial pressure (PCO2),alkali residual (BE) and pH were significantly improved at 12 h after treatment,and lasted until 36 h after treatment;the differences were statistically significant (P＜0.05).In the experimental group,blood calcium increased,creatinine,urea nitrogen and blood sugar level decreased significantly,blood potassium and blood sodium level were stable within 12 h after treatment,and the difference was statistically significant,compared with the control group (P＜0.05).In the experimental group,the alanine aminotransferase (ALT) decreased significantly after 36 h of treatment,and the difference was statistically significant(P＜0.05).There was a significant difference between the experimental group and the control group in the hematocrit (HTC) and white blood cell count (WBC) before treatment (P＜ 0.05).The platelet count (PLT) in the experimental group decreased within 12 h,and the difference was statistically significant before treatment(P＜0.05).Conclusion Compared with the conventional treatment,the therapeutic effect of CRRT on SAP is more significant.The improvement of the physiological and biochemical indexes of the patients is obvious,which is of great significance to the prognosis of the patients.

Effects of FEA from Polygonum hydropiper L. on Biochemical Indexes and Inflammatory Cytokines in Mice with Endotoxemia Induced by Lipopolysaccharide / 中国中医药信息杂志

Objective To observe the effect of flavonoids ethyl acetate(FEA)from Polygonum hydropiper L.on biochemical indexes and inflammatory cytokines in mice with endotoxemia; To expore the mechanism. Methods Total flavonoids in the whole plant of Polygonum hydropiperum L. were extracted by enzymolysis-ultrasonic coupling method. The FEA part were obtained by extracting and separating, followed with macroporous resin purification and enrichment. The animal model of endotoxemia was established by stimulation of lipopolysaccharide (LPS) in mice. Experimental mice were divided into blank group, model group, FEA high-, medium-, and low-dose groups. Each administration group was given the corresponding concentration of herb liquor. The concentration of malondialdehyde (MDA) and myeloperoxidase (MPO) in intestinal tissue, total antioxidant capacity (T-AOC), superoxide dismutase (T-SOD) activity and glutathione peroxidase (GSH-Px) activity in liver tissue, glutathione (GSH), lysozyme (LZM) and acid phosphatase (ACP) in serum were determined. The contents of tumor necrosis factor-α (TNF-α), interferon (IFN)-α, IFN-γ and interleukin-2 (IL-2) in lung tissue were detected by RT-PCR. Results Compared with blank group,the levels of MDA, MPO in intestinal tissue and serum ACP of model mice were increased, while T-AOC, T-SOD, GSH-Px in liver tissue, serum GSH and LZM levels were decreased; TNF-α in serum, intestinal and liver tissues were increased, the expressions of TNF-α, IFN-α, IFN-γ and IL-2 mRNA in lung tissue were increased. Compared with the model group, the levels of MDA, MPO in intestinal tissue and serum ACP were decreased in all dose of FEA groups;The levels of T-AOC, T-SOD, GSH-Px in liver tissue, serum GSH and LZM of FEA medium and high-dose groups were increased. The content of TNF-α in mice serum, intestinal and liver tissues of all dose of FEA groups were significantly reduced, and the expressions of TNF-α, IFN-α, IFN-γ and IL-2 mRNA in lung tissues were significantly decreased. The pathological morphology of mice lung, ileum and colon tissues of FEA high-dose group were significantly ameliorated than model group. Conclusion FEA can attenuate inflammation injury of endotoxemia mice induced by LPS, which has protective effects for organism.

Application of percutaneous transhepatic cholangial drainage in the treatment of bile duct obstruction with malignant hilar bile duct carcinoma / 局解手术学杂志

Objective To analyze the therapeutic effect of percutaneous transhepatic cholangial drainage (PTCD) in the treatment of bile duct obstruction in patients with malignant hilar bile duct carcinoma,and to discuss the clinical application and practical value of PTCD.Methods A total of 55 patients with malignant biliary obstruction were divided into the PTCD group (30 cases who recieved percutaneous transhepatic cholangial drainage) and the control group (25 cases who recieved endoscopic stent implantation).Observed the preoperative and postoperative biochemical indexes of PTCD group,including serum total bilirubin (TB),serum direct bilirubin (DB),serum alanine aminotransferase (ALT) and serum glutamic acid amino turn shift of aspartate aminotransferase(AST) and serum alkaline phosphatase(AKP).Compared the effect rate and postoperative survival time of the two groups through postoperative follow-up.Results The TB,DB,ALT,AST and APK of PTCD group one week after operation changed obviously compared with the relative index before opreation with statistically significant differences (P<0.05), which indicated a significant improvement of biochemical indicators.The treatment efficiency of the PTCD group and the control group were 83.3% and 64.0% respectively, and survival time of the two groups were(7.5±2.6)months and(4.8±2.8)months respectively.Results of the PTCD group was significantly better than that of the control group,and the differences were statistically significant(P<0.05).Conclusion All the patients with PTCD get better biochemical indicators and longer postoperative survival time,and the interventional therapy PTCD can be used as an effective clinical treatment method for bile duct obstruction with malignant hilar bile duct carcinoma.

Mensurement and analysis of biological characteristics of C57BL/6J mice and their offsprings after frozen-thawed embryo transfer of C57BL/6J mice / 中国比较医学杂志

Objective To investigate the changes of average body weight gain and serum biochemical indexes of C57BL/6J mice (B6 mouse) and their offspings after frozen-thawed embryo transfer of B6 mice.Methods The mice were divided into three groups in this study.In the experimental group I (E-I,30 males and 20 females),2-cell embryos after in-vitro fertilization were collected,and cryopreserved by EFS method,then obtained the offsprings after transplantation of the recovered embryos to oviduct of recipient mice (ICR mouse).In the experimental group II (E-II,26 males and 17 females),when the mice from E-I grew to maturity,the offsprings were obtained from natural mating of mice from E-I.In the control group (20 males and 20 females),the offsprings came from conventional feeding and natural mating.The three groups of mice were raised to 16 weeks old,weighing the body weight at a regular time intervals,and the serum biochemical indexes were obtained from 16-week-old mice.Then the changes of average body weight and serum biochemical indexes of the mice were analyzed.Results The average body weight of E-I mice was significantly higher than that of control group at each week-age (P<0.01).The average body weight of E-II female mice was significantly higher than that of the control group in 12-16-week old mice (P<0.01),but the average body weight of E-II male mice showed no significant differences compared with the control group except for few weeks.The serum biochemical indexes of E-I and E-II mice were changed in all items except for AST,TP and Ca.Conclusions There are some effects on the average body weight gain and serum biochemical indexes of C57BL/6J mice and their offspings after frozen-thawed embryo transfer.

Clinical observation on the differences of liver function indexes in acute left heart failure and congestive heart failure / 中国生化药物杂志

Objective To compare the differences of liver function indexes in patients with acute left heart failure and congestive heart failure , and to provide the basis for the diagnosis of heart failure .Methods 142 cases of heart failure from March 2014 to December 2015 in our hospital were selected and divided into 68 cases of acute left heart failure group and 74 cases of congestive heart failure group.The differences in liver function tests were compared between the two groups by comparing the differences in the indexes of heart failure, liver blood biochemistry, general biochemical indexes and late follow-up data.Results Compared with the indexes of heart failure, there were no significant differences in ejection fraction, NT-proBNP, history of heart failure>one years, and the number of patients with right heart failure, the difference was statistically significant (P<0.05).Comparison of liver blood biochemistry: patients with acute left heart failure were alanine aminotransferase, aspartate amino transferase, albumin increased, the difference was statistically significant (P<0.05), and glutamyl aminotransferase, total bilirubin, direct bilirubin, indirect bilirubin decreased, the difference was statistically significant (P<0.05).There was no significant difference between the two groups of patients with alkaline phosphatase and total protein.Compared with the general biochemical indexes, increased triglyceride levels in patients with congestive heart failure group, the difference was statistically significant (P<0.05), there was no significant difference in other indexes.Conclusion AHF patients with ALT, AST increased, CHF with GGT, TBIL, DBIL, IBIL increased, ALB decreased mainly.

Biochemical indices of red blood cells in additive solution leukocytes reduced preserved in navy ship force during a long voyage / 军事医学

Objective To explore the influence on and changes in the biochemical indices of red blood cells(RBCs)in additive solution leukocytes reduced preserved in navy ship force on a long voyage.Methods According to the Requirement of Health Examination for Blood Donors(GB 18467 -2011),RBCs in additive solution leukocytes reduced were prepared from 10 healthy voluntary blood donators one day before sailing.Each blood sample was divided into two parts,one in test group and another in control group.All the groups had samples taken for the biochemical index detection after 1,3,5,7,14 and 21 days of sailing respectively.Results ①The change in total protein(P=0.235)and albumin (P=0.119)concentration was not obvious,and the difference between the two groups was not significant.②The change in total creatinine(P=0.001)and uric acid(P=0.001)concentration was obvious, but the difference between the two groups was not significant.③The change in total cholesterol(P=0.354)concentration was not obvious,but the change in triglycerides(P=0.005)concentration was significant.The difference between the two groups was not significant.④The concentration of lactate dehydrogenase, hydroxybutyrate dehydrogenase and creatine kinase increased with the time of preservation(P<0.001).The difference between the two groups was not significant.⑤The interaction between grouping effect and time effect had no significant influence on the concentration of osmolarity(OSM)(P=0.968)and glucose(Glu) (P=0.406).Between the two groups,the difference of concentrations of OSM(P=0.569)and Glu(P=0.115)was not significant.Conclusion Under the 4 class sea conditions, a long voyage has some impact on the storage of RBCs in additive solution leukocytes reduced,as in the conventional blood storage refrigerator(4 ±2)℃.The results of this study have important clinical implications for our further study of marine blood support.

Research on Saliva Biochemical Changes about “Normal Constitution - Kidney Deficiency Constitution - Kidney Deficiency Syndrome” / 中国中医药信息杂志

Objective To investigate the changes of saliva biochemical parameters in the dynamic evolution of “normal constitution - kidney deficiency constitution - kidney deficiency syndrome”.Methods Totally 24 male and female SD rats of 3 months old (12 male rats and 12 female rats) were put in packet pair cage. Pregnant rats were randomly divided intoⅠ andⅡ groups, 6 rats in each group.Ⅰ group was without intimidation during pregnancy, with normal feeding, and then after the birth, 20 rats were randomly selected as normal control group and kidney deficiency syndrome model group, 10 rats in each group, half male and half female.Ⅱ group, from gestation day 2, was given intimidation until postnatal. 10 half male and half female rats were randomly selected as model group of kidney deficiency constitution. Rats in each group received normal feeding conditions for 6 weeks. The kidney deficiency syndrome model group was given intramuscular injection of hydrocortisone 25 mg/kg body weight every day, while normal control group and kidney deficiency constitution model group were injected with the same amount of normal saline for 10 consecutive days. The changes of salivary biochemical parameters were observed in each group of rats.Results Compared with the normal control group, salivary pH in the kidney deficiency constitution group and kidney deficiency syndrome group significantly increased (P<0.01). CK, ALT, AST, ALP levels in the kidney deficiency constitution group were reduced, but the contents of K, P, TP, AMY increased. The levels of CK, ALT, AST, ALP and LDH in the kidney deficiency syndrome decreased, while the contents of K, P, TP, and AMY increased (P<0.05,P<0.01). Compared with the kidney deficiency constitution group, the content of LDH in the kidney deficiency syndrome group decreased (P<0.05).Conclusion Rat saliva composition changes did occur in the kidney deficiency constitution group and kidney deficiency syndrome group.

Application analysis of tumor markers CEA,CA125,CA199 in differential diagnosis of malignant pleural effusions / 国际检验医学杂志

Objective To explore the difference of serum and pleural effusion CEA ,CA125 and CA199 levels in benign and ma‐lignant pleural effusions to accumulate the practical experience for clinical diagnosis .Methods 125 patients with pleural effusion in this hospital from February 2010 to March 2014 were performed the retrospective analysis and divided into the benign group(49 ca‐ses) and malignant group(76 cases) according to the nature of the fluids .The etiology composition ,pleural effusion and serum tumor markers levels were recorded in the two groups and at the same time the diagnosis efficiency of the tumor markers in pleural effusion was calculated .Results The specificity of the combined detection of CEA ,CA125 and CA199 for diagnosing benign and malignant pleural effusion was 90 .6% ,the sensitivity was 95 .1% ,the positive predictive value was 90 .2% ,negative predictive val‐ue was 95 .3% and accuracy was 92 .7% ,which were higher than those of the single index detection and any 2‐marker combined de‐tection .Conclusion The combined diagnosis of CEA ,CA125 and CA199 in pleural effusion conduces to the assisted diagnosis of be‐nign and malignant pleural effusion .

Effect of bortezomib combined with dexamethasone on biochemical indexes in patients with multiple myeloma bone disease / 中国生化药物杂志

Objective To explore the clinical effect and biochemical index changes of bortezomib combined with dexamethasone in multiple myeloma bone disease.Methods A total of 40 cases of multiple myeloma patients were randomly divided into experimental group (20 cases) and control group (20 cases), two groups of patients were treated with zoledronic acid, the experiment group adopted the bortezomib and dexamethasone treatment scheme, control group received dexamethasone+vincristine+epirubicin scheme.After 3 courses of treatment,compared the pain relieving degree,clinical efficacy and adverse reactions incidence, and analysed serum calcium, phosphorus, DDK1, RANKL, TRACP-5b and ALP levels of two groups.Results After the end of chemotherapy,bone pain in two groups was significantly relieved,and the pain relieving degree experimental group was significantly better than the control group (P<0.05).Total effective rate in experimental group was 95.0%, significantly higher than that in control group 65.0%(χ2 =5.625,P=0.018).The adverse reaction rate had no significant difference between two groups.The of calcium, phosphorus after chemotherapy, DDK1, RANKL, TRACP-5b levels in experimental group were significantly lower than those in control group, ALP was significantly higher than that of control group (P<0.05).Conclusion Bortezomib in combination with dexamethasone can significantly improve the curative effect of multiple myeloma,and its mechanism may regulate DDK1, RANKL, TRACP-5b levels,and balance the osteolytic and osteoblastic process.

Effect of midazolam combined with fentanyl as adjuvant therapy on inflammatory factors and biochemical indexes in children with serious hand-foot-mouth disease with mechanical ventilation / 中华传染病杂志

Objective To investigate the effect of midazolam combined with fentanyl as adjuvant therapy on inflammatory factors and biochemical indexes in children with serious hand-foot-mouth disease with mechanical ventilation .Methods One hundred and thirty children with serious hand-foot-mouth disease treated with mechanical ventilation were selected at Maternal and Child Health Care Hospital from January 2010 to January 2014 . The patients were divided into two groups according to the treatment regimen :58 cases treated with midazolam for sedation and analgesia as control group and 72 cases treated with midazolam combined with fentanyl as observation group . Inflammatory factors (interleukin-6 , interferon-γ ,high-sensitivity C-reactive protein and tumor necrosis factor-α) and biochemical indices (albumin ,alanine transaminase [ALT] ,aspartate transaminase [AST] ,alkaline phosphatase ,glutamate transpeptidase [γ-GT] and fasting blood glucose) before drug exposure and on withdrawal were compared between two groups .Adverse reactions were analyzed in the two groups .Continuous variables were compared using two-sample t-test , while categorical variables were compared using chi-square test . Results Interleukin-6 ,interferon-γ, high sensitive C reactive protein and tumor necrosis factor-α on withdrawal decreased significantly in both groups than those before drug exposure (all P0 .05) .However ,in observation group ,fasting blood glucose level decreased significantly on drug withdrawal compared with that before drug exposure ([5 .17 ± 0 .28] vs [10 .31 ± 1 .39] mmol/L ,t=46 .237 ,P=0 .000) ,and that was also lower than control group ([5 .17 ± 0 .28] vs [5 .85 ± 0 .34] mmol/L ,t=4 .372 ,P=0 .000) .Incidence of adverse reactions in observation group was significantly lower than control group (15 .3% vs 32 .8% ,χ2=4 .707 ,P=0 .030) . Conclusions Midazolam combined with fentanyl as adjuvant therapy is helpful to improve blood glucose , stabilize biochemical indices and reduce inflammation factor secretions in children with serious hand-foot-mouth disease with mechanical ventilation .This therapy is safe and worthy of clinical use .

The diagnostic value of cerebrospinal fluid biochemical indexes in central nervous system infection / 国际检验医学杂志

Objective To explore the diagnostic value of cerebrospinal fluid(CSF) biochemical indexes in central nervous system (CNS) infection .Methods The results of CSF culturing and detection of biochemical indexes including glucose(Glu) ,lactate dehy‐drogenase(LDH) ,chloride ion(Cl) and protein(PRO) were collected from the First Affiliated Hospital of Xi′an Jiaotong University from September to December 2013 .Taking the results of CSF culturing as golden standard ,the biochemical indexes were analyzed by using receiver‐operating characteristic(ROC) curve for single index and combined indexes .Results A total of 157 CSF speci‐mens were collected ,including 39 culture‐positive specimens and 118 culture‐negative specimens .Areas under ROC curve(AUC) of Glu ,LDH ,Cl and PRO were 0 .653 ,0 .633 ,0 .626 ,0 .671 ,respectively .The AUCs of combined detection of these biochemical inde‐xes were higher than those of single index ,and the AUC of combined detection of these four indexes was the largest ,up to 0 .694 , but all were less than 0 .7 .Conclusion Glu ,LDH ,Cl and PRO all could be independent biochemical indexes for diagnosing CNS in‐fection ,among which PRO has the highest diagnostic value .And combined detections of these indexes are better than single detec‐tions ,especially combination of four indexes ,which is the strongest indicator ,but accuracy is low whether single or combined detec‐tion .More sensitive and specific biomarkers of CSF should be explored for rapid diagnosis of CNS infection .

Research on blood biochemical indexes in surgical patients with massive blood transfusion at Viet Duc hospital

Background: Massive blood transfusion is common in surgery: trauma, complicated surgery, organ implantation. Stored blood contains much lactate, ammonia, products of metabolism. These products have negative effects on patient\u2019s metabolic function which may be a cause of death. Objective: To study the change of blood biochemical indexes in surgical patients with massive blood transfusion at Viet Duc hospital. Subjects and method: A prospective study was conducted in the adult patients who had emergency and operation at Viet Duc hospital, from December/2004 to August/2006. All of them were transfused over 3000 ml of blood within 24 hours (pre-operation, intra-operation and post-operation). Results: During the period of study, there were 70 surgical patients with massive blood transfusion: men were more than woman (64%) and the mean age: 38\xb117. The survival rate was significantly improved (57%). The average transfused blood per patient per 24 hours was 3995ml\xb11067ml. There was a decrease in the value of pH, acid-base and HCO3 (the average pH value 7.218\xb10.181, lowest value 6.7). The average value of pH and HCO3\xac was higher in the survival patients than died patients (p<0.05). The mean level of K+ was 3.8\xb10.96mml/l. The increase in the level of K+ had a positive association with the amount of transfused blood. The mean level of Ca++ \xac\xacdecreased under the normal level (0.98mml). Conclusion: It should monitor blood gas and electrolytes continuously in surgical patients with massive blood transfusion in order to identify disorders soon for timely treatment.

Changes in biochemical indexes after interim overload exercise and their significances / 吉林大学学报(医学版)

Objective To study the influence of interim overload exercise on health and it's biochemical mechanism.Methods 45 Wistar rats were randomly divided to three groups(n=15):no exercises group(control,C),normal exercise group(NE,the rats ran in the animal running machines at 15 m?min-1for 5 d in one week,exercise time was 30 min?2,and rested for 10 min after ran for 30 min),overload exercise group(OE,the rats exercised 2 bouts,2 d in consecutive in one week,exercise time was 100 min?2,and they rested for 10 min after ran for 100 min).After training for 8 weeks,the rats in three groups were sacrificed and 15 biochemical indexes in blood of rats were determined.Results Compared with C and NE groups,CK,LDH,and ALT increased obviously(P